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purH [2019-06-24 08:43:13]
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purH [2019-06-24 08:43:13]

phosphoribosylaminoimidazole carboxamide formyltransferase
Locus
BSU06520
Isoelectric point
5.13
Molecular weight
55.57 kDa
Protein length
512 aa Sequence Blast
Gene length
1539 bp Sequence Blast
Function
purine biosynthesis
Product
phosphoribosylaminoimidazole carboxamide formyltransferaseand inosine-monophosphate cyclohydrolase
Essential
no
E.C.
2.1.2.3
Synonyms
purJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides

    • Gene

    Coordinates
    708,594 710,132

    Phenotypes of a mutant

  • more resistant than wild-type to zinc limitation when grown on rich medium (LB), this is blocked by simultaneous deletion of purB (reason: accumulation of ZTP that stimulates the binding of ZagA to FolE, and thus folate biosynthesis during zinc limitation) PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • 10-formyltetrahydrofolate 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to Swiss-Prot)

    • Protein family

  • purH family (according to Swiss-Prot)

    • Structure

  • 1ZCZ (from Thermotoga maritima, 36% identity, 54% similarity) PubMed

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • G-box: termination, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon

    • Regulation

  • expression activated by glucose (4.4 fold) PubMed
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    Biological materials

    Mutant

  • BKE06520 (purH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA
  • BKK06520 (purH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA

    • References

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